Effects of ECM protein micropatterns on the migration and differentiation of adult neural stem cells
Sunghoon Joo, Joo Yeon Kim, Eunsoo Lee, Nari Hong, Woong Sun & Yoonkey Nam
This is an outcome of our on-going collaboration with Prof. Woong Sun's lab in Korea University (고려대학교 해부학 교실, 선웅 교수). In this work, Sunghoon (KAIST, NEL) and Joo Yeon (Korea University College of Medicine) applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how aNSCs respond to the various spatial distribution of laminin, one of ECM proteins enriched in the aNSC niche. They found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, they discovered a new way of separating neurons and astrocytes differentiated from adult neural stem cells. To the best of our knowledge, this is the first time to investigate the differential cellular responses of aNSCs on ECM protein (LN) and cell adhesive synthetic polymer (PDL) using surface micropatterns.
Source: Joo et al., Scientific Reports 2015 (Link)