Tissue-based metabolic labeling of polysialic acids in living primary hippocampal neurons.
Kang Kyung Tae, Joo Sung Hoon, Choi JY, Geum S, Hong S, Lee S, Kim YH, Kim S, Yoon M, Nam Y*, Lee K*, Lee H (link)*, Choi IS (link)*
In this work, we developed a tissue-based strategy for metabolic incorporation of a chemical reporter to primary neurons. We let an unnatural monosaccharide be metabolized by hippocampal tissues before dissociation into individual cells, and thereby, we could eliminate cytotoxicity. We used this method to describe, for the first time to our knowledge, the real-time distribution of polysialic acids on the membranes of neurons. Current member Sung Hoon Joo, previous member Dr. Kyungtae Kang, and JY Choi from Choi lab (link) did a great collaboration to make this happen.
Article abstract (Link)