BRIC 한빛사에 주성훈 학생의 최근 PNAS 논문 인터뷰가 실렸습니다. 인터뷰 링크
Sunghoon interviewed with BRIC concerning his work published in PNAS .
Tissue-based metabolic labeling of polysialic acids in living primary hippocampal neurons.
Kang Kyung Tae, Joo Sung Hoon, Choi JY, Geum S, Hong S, Lee S, Kim YH, Kim S, Yoon M, Nam Y*, Lee K*, Lee H (link)*, Choi IS (link)*
In this work, we developed a tissue-based strategy for metabolic incorporation of a chemical reporter to primary neurons. We let an unnatural monosaccharide be metabolized by hippocampal tissues before dissociation into individual cells, and thereby, we could eliminate cytotoxicity. We used this method to describe, for the first time to our knowledge, the real-time distribution of polysialic acids on the membranes of neurons. Current member Sung Hoon Joo, previous member Dr. Kyungtae Kang, and JY Choi from Choi lab (link) did a great collaboration to make this happen.
Article abstract (Link)
Agarose-Assisted Micro-Contact Printing for High-Quality Biomolecular Micro-Patterns
Min Jee Jang, Yoonkey Nam
This work reports a novel surface modification of a PDMS stamp for micro-contact printing of biomolecules. We developed a method to form a thin agarose-hydrogel layer on a PDMS stamp, which greatly enhanced the quality of printed micropatterns in terms of uniformity and ink transfer. This is the second paper on a micro-contact printing from NEL. (Link)